Does Biotin Increase Testosterone? Know Science

Does Biotin increase testosterone

Known as B7, Biotin is one of the B vitamins which has gained a lot of attention lately because of the various wellness benefits it is believed to offer, particularly, in the fields of skin and hair. However, today’s article is all about whether biotin increases testosterone levels. While it is said to influence the testosterone response in a certain way, whether it affects positively or negatively is the subject of discussion.

Biotin is essential for numerous biochemical processes associated with the digestive system, brain functioning, blood sugar control, muscles, and tissues among many others. These are in some way also connected with testosterone health. In order to get more specific answers, we did thorough research on this subject, went through the work of various research groups, and here’s what we found:

Important Points To Know
  • One of the studies conducted on animal subjects showed that biotin has a positive influence on testosterone production.
  • A deficiency of biotin can result in improper functioning of testicles in animals.
  • The excessive presence of biotin in the body may mislead test results or lead to inappropriate testosterone measurement.
  • More thorough studies and analyses are needed to establish biotin’s potential in testosterone synthesis.

How Does Biotin Affect Testosterone Levels?

How Does Biotin Affect Testosterone Levels

Vitamin B in general is believed to have a direct link with testosterone health because of which many supplements have B vitamin as one of the key additions in their ingredient list. However, it is important to note that these testosterone boosters usually go for vitamin B complex, which is a combination of various vitamins from the B group that may include B3, B5, B6, B7, and/or B12. But when it comes to individual impact, how effective vitamin B7 or biotin is, is the concern here.

A study conducted quite a while ago on animal subjects found that low levels of biotin or a deficiency can have a negative impact on testicular health leading to a decline in testosterone. In addition to that, the luteinizing hormone (LH) released by the pituitary gland was also found to be significantly low in biotin-deficient subjects. For those who are not aware, LH is responsible for triggering testosterone production and a decrease in this hormone may also have affected testosterone.

Furthermore, the study also found that when the subjects were supplemented or treated with biotin, testosterone levels were regulated. A testosterone treatment, however, without biotin supplementation had no impact on the deficiency condition.

One of the recent studies conducted in 2022 also found similar results. The research findings indicate that high doses of biotin supplementation raised serum testosterone in male mice, in addition to improving their testicular functioning.

Now, while the above studies do exhibit a positive correlation between biotin and testosterone, there are a couple of points to note here-

Firstly, both the studies were conducted on animal subjects and further research is required to find out and evaluate its impact on humans. There is no guarantee that biotin will have a similar kind of influence on testosterone in humans.

Secondly, one of the things that can be inferred from the above studies is that, even if biotin has a similar effect on humans, it may only work for those who have a deficiency of this vitamin. In any case, more concrete research and clinical trials are needed to determine the association between biotin and testosterone in humans.

Effects Of Biotin Supplementation

Effects of Biotin Supplementation

Some of the reports related to biotin and testosterone have indicated that biotin has the potential to delude certain blood test results. With the increase in popularity of this vitamin for its potential benefits, biotin supplementation has become common among many individuals.

However, there are studies and reports which show that the excessive presence of biotin as a result of supplementation can be harmful in different ways. For example, it can interfere with the blood during immunoassay tests in which biotin is used as one of the test components.

Biotin has the ability to link itself with certain molecules or substances because of which it is used in various tests. Excessive amounts of this vitamin through supplementation can inhibit its binding ability. This may result in incorrect evaluation leading to inaccuracy in test reports and wrong treatment.

All that said, the presence of biotin, may not have a fatal impact, however, it is always a good idea to consult a healthcare practitioner before taking biotin supplements especially if you are working on raising your testosterone levels.

Supplementary Benefits Of Biotin

While most of the studies about biotin’s benefits on testosterone have been on animals, one of the research findings suggests that biotin can act as a highly sensitive ‘testosterone tracer’ which can be beneficial in measuring the hormone levels in humans. The research evaluated both normal and low testosterone levels in testosterone-treated as well as untreated hypogonadal men. The results reportedly turned out to be impressively accurate.

It should be noted that the test only confirmed its ability to detect testosterone levels more efficiently and rapidly in hypogonadal men which can be useful in deciding the next course of action faster. However, it has no direct contribution to raising testosterone levels.


It is difficult to conclude whether biotin can actually increase testosterone levels in men because of the limited availability of evidence and findings. The currently available resources which demonstrate a connection between testosterone and biotin are experiments conducted on animals which are insufficient to determine its influence on humans. Therefore, in order to get a convincing answer, we need more clinical trials with human volunteers.

That said, biotin can be a crucial part of your diet because of its other health perks. When taken along with a naturally-formulated testosterone booster, it can effectively accelerate metabolic function which can indirectly aid testosterone synthesis. Taken in combination with other natural ingredients can help balance the vitamin thus removing excess of it.